CORE FACILITIES AND RESOURCES

Histology

The Histology Core supports the preparation, embedding, sectioning, and storage of biological specimens for observation and analysis by light microscopy.

The Core provides for fresh, frozen, and paraffin specimen processing. Equipment and services for routine staining are available, including H&E, PAS, and MT. The core can also assist with specimen preparation and provide advice for specialized staining techniques such as immunohistochemistry and in situ hybridization.

Specimens can be examined at all stages of preparation by standard light microscopy and phase contrast microscopy.

The Histology Core stores tissue specimens and maintains a specimen database.

The Histology Core is supported by NEI Core Center Grant P30 EY014801. Please cite this grant number on all publications that make use of this core.

Leadership & Management

Jean-Marie Parel, PhD directs the Histology Core. Dr. Parel is appointed to the Henri and Flore Lesieur Chair in Ophthalmology.

Magda Celdran provides histology services to MCVR Investigators.

Magda Celdran is the Histology Technician for the Histology Core.

Sander R. Dubovy, MD serves as consultant to the Histology Core Facility. Dr. Dubovy serves as Assistant Professor of Clinical Ophthalmology and holds a secondary appointment in the Department of Pathology. He also serves as Director of Bascom Palmer’s Pathology Laboratory and as the Medical Director of the Florida Lions Eye Bank, both located within the Bascom Palmer Eye Institute.

Location

The Histology Core is located on the 4th floor of the McKnight building.

The lab was newly-renovated in 2004. It was carefully designed for efficient work flow. It contains a built-in Labconco fume hood (with blower, cabinet and work surface for staining apparatus), and 2 chemical sinks.

Equipment  

• ATP™ Tissue Processor (# ATP1-120, Triangle Biomedical Sciences, Inc.)
• 2 Microtome Cryostats (International Equipment Company – Minotome)
• Leica Jung knife sharpener
• 2 micro optics Microtomy (Leitz #1512)
• Spencer binocular microscope (AO #926963)
• Lancer Vibratome 1000 and Vibratome 1000 Plus
• Illuminated Tissue Flotation Bath (# 80086-990, VWR)
• Paraffin Dispenser (# PD-120, Triangle Biomedical Sciences, Inc)

[back to top]